Part:BBa_K1088000:Design

1-deoxyxylulose-5-phosphate synthase (Dxs) enzyme from MEP pathway isolated from B. subtilis 168

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 1063
  Illegal EcoRI site found at 1720
  Illegal PstI site found at 1121
  Illegal PstI site found at 1567
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 1063
  Illegal EcoRI site found at 1720
  Illegal PstI site found at 1121
  Illegal PstI site found at 1567
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 1063
  Illegal EcoRI site found at 1720
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 1063
  Illegal EcoRI site found at 1720
  Illegal PstI site found at 1121
  Illegal PstI site found at 1567
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 1063
  Illegal EcoRI site found at 1720
  Illegal PstI site found at 1121
  Illegal PstI site found at 1567
  Illegal NgoMIV site found at 1020
  Illegal AgeI site found at 913
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 862
  Illegal SapI.rc site found at 1561
  

Design Notes

we changed TTG start codon to ATG to improve translation efficiency. We performed 4 silent site directed mutageneses to remove 2 EcoR1 and 2 Pst1 restriction sites

Source

B. subtilis 168

References